Monoclonal Anti-Myogenin antibody produced in mouse
SIGMA/M5815 - clone F12B, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD01322371
Product Type: Chemical
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| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | F12B, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 34 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using rhabdomyosarcoma tissue sections |
| immunoprecipitation (IP): 2 μg using 1 mg protein lysate | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1 μg/mL | |
| UniProt accession no. | P15173  |
| Application: | Monoclonal Anti-Myogenin antibody produced in mouse has been used in immunohistochemistry, immunofluorescence and western blotting. |
| Biochem/physiol Actions: | Myogenin acts as a biomarker in rhabdomyosarcoma (RMS). It has a role in developmental processes, such as hematopoiesis, neurogenesis, myogenesis, tumor cell invasion and metastasis. |
| Biochem/physiol Actions: | Myogenin acts as a regulator during myoblast and skeletal muscle differentiation. It is considered as a definite marker for skeletal muscle differentiation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myogenin belongs to the basic helix-loop-helix (bHLH) superfamily. |
| General description: | Myogenin is a transcription factor present in the nucleus. It possesses an amino-acid rich region and a helix-loop-helix structure. The gene encoding it is localized on human chromosome 1q31-41 and has three exons. |
| Immunogen: | rat recombinant myogenin (amino acids 30-224). |
| Physical form: | Solution in phosphate buffered saline containing 0.08% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |