IgM Isotype Control from murine myeloma
SIGMA/M5909 - clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin
Synonym: Mouse IgM Isotype Control from myeloma
MDL Number: MFCD00164813
Product Type: Chemical
Illustration of IgM
| antibody form | purified immunoglobulin |
| biological source | mouse |
| clone | MOPC 104E, monoclonal |
| concentration | 200 μg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgMλ |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| Application: | IgM isotype control antibodies are suitable for use in immunohistochemistry . |
| Application: | IgM Isotype Control clone MOPC 104E is recommended as a negative control in flow cytometry. Working Concentration: equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry. Equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry. |
| Application: | IgM Isotype Control from murine myeloma has been used: • in chromatin immunoprecipitation (ChIP) assay • LABScreen assays • immunofluorescent staining |
| Application: | Mouse myeloma proteins tested for use as controls in flow cytometry. |
| Biochem/physiol Actions: | The specificity of staining by monoclonal antibodies to mouse CD antigens should be verified by establishing the non-specific reagent binding to the target cell population. It is recommended that a concentration and isotype matched mouse myeloma immunoglobulin be included as a negative control for each monoclonal antibody reagent used in the immunoassay procedure. Sigma Mouse Isotype Controls are supplied to match the concentration of Sigma purified and/or conjugated monoclonal antibodies to CD antigens. DUAL-TAG controls contain 100 μg of each conjugate. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory . This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice . IgM isotype control antibody is specific for anti-mouse whole serum and anti-mouse IgM. The product does not react with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3. |
| General description: | Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays. |
| General description: | The purified IgM Isotype Control clone MOPC 104E preparation is non-reactive with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3. When evaluated in flow cytometry, the IgM Isotype Control did not stain human peripheral blood lymphocytes (PBLs). A FITC Goat anti-Mouse IgM (μ-chain specific), Affinity Isolated Antibody (Product No. F 9259) along with 1 μg of the product was incubated with human PBLs and then evaluated by flow cytometry. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |