Anti-MIB1 (C-terminal) antibody produced in rabbit
SIGMA/M5948 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-
Product Type: Chemical
Immunoblotting Lysates of various cell lines were separated on SDS-PAGE and blotted with 1 μg/mL Rabbit Anti-MIB1 (C-terminal) (Cat. No. M5948). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545), and a chemiluminiscence substrate. Lanes: 1. A431; 2. A549; 3. 293-T over expressing human MIB1; 4. PC-12
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~110 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1 μg/mL using lysates of A549 or PC12 cells. |
| UniProt accession no. | Q86YT6  |
| Application: | Anti-MIB1 (C-terminal) antibody produced in rabbit has been used in:immunofluorescence, immunoprecipitation , western blotting immunohistochemistry |
| Application: | Anti-MIB1 (N-terminal) antibody produced in rabbit has been used for immunofluorescence. |
| Biochem/physiol Actions: | MIB1 is critical for mammalian development and cellular differentiation. It also plays a role in neuronal morphogenesis partially through physical interaction with cyclin-dependent kinase 5 (CDK5)/p35. Knockout of MIB1 in mice results in embryonic mortality with many defects observed in somitogenesis, neurogenesis, vasculogenesis and cardiogenesis. |
| Biochem/physiol Actions: | Mindbomb homolog 1 (MIB1) aids in the ubiquitination and internalization of Notch ligands such as the protein δ. It also acts as a positive regulator in the activation of nuclear factor-κB (NF-κB). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MIB1 (Mindbomb homology 1) are E3 ubiquitin ligases. It is abundantly expressed in both embryos and adult tissues, whereas MIB2 is highly expressed in adult tissues. |
| General description: | Mindbomb homolog 1 (MIB1) is an ubiquitin ligase which possesses two substrate recognition domains, ankyrin repeats and an amino terminal zinc finger domain. |
| Immunogen: | synthetic peptide corresponding to amino acids 924-939 of human MIB1, conjugated to KLH. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |