Monoclonal Anti-MYOD1 antibody produced in mouse
SIGMA/M6190 - clone 5.2F, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Myogenic Differentiation Antigen 1
MDL Number: MFCD01323984
Product Type: Chemical
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| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5.2F, monoclonal |
| concentration | 1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen 34 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human, rat, chicken, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL | |
| immunohistochemistry (frozen sections): 2-4 μg/mL | |
| immunoprecipitation (IP): 2 μg using 1 mg protein lysate | |
| western blot: 1 μg/mL (reacts with the ~45 kDa protein) | |
| UniProt accession no. | P15172  |
| Application: | Monoclonal Anti-MYOD1 antibody produced in mouse has been used in: • immunofluorescence staining at a 1:50 dilution • western blotting • immunostaining at a 1:300 dilution |
| Biochem/physiol Actions: | Myogenic differentiation antigen 1 (MYOD1) maybe involved in recruitment of enzymes like acetyltransferases and methyltransferases to myogenic enhancers in the human genome. It takes part in the regeneration of muscles and mediates muscle cell differentiation by activating cell cycle arrest. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myogenic differentiation antigen 1 (MYOD1) is a nuclear protein which is expressed in skeletal muscles. It is part of the basic helix-loop-helix (bHLH) family of transcription factors. The gene encoding it is localized on human chromosome 11. |
| Immunogen: | recombinant mouse MyoD1 protein. |
| Physical form: | Solution in phosphate buffered saline containing 0.08% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |