Monoclonal Anti-Maltose Binding Protein antibody produced in mouse
SIGMA/M6295 - clone MBP-17, ascites fluid
Synonym: Monoclonal Anti-Maltose Binding Protein; Maltose Binding Protein Antibody; Maltose Binding Protein Antibody - Monoclonal Anti-Maltose Binding Protein antibody produced in mouse; Anti-MBP
Product Type: Chemical
Immunoblotting
Maltose Binding Protein (MBP) was separated on SDS-PAGE and blotted with Monoclonal Anti-Maltose Binding Protein (Cat. No. M6295). The antibody was developed with Goat Anti-Mouse IgG (Fab), AlkPhos Conjugate (Cat. No. A1293) and NBT/BCIP substrate.
Lanes
A: Antibody dilution 1:8,000
B: Negative control (only secondary antibody)
Maltose Binding Protein (MBP) was separated on SDS-PAGE and blotted with Monoclonal Anti-Maltose Binding Protein (Cat. No. M6295). The antibody was developed with Goat Anti-Mouse IgG (Fab), AlkPhos Conjugate (Cat. No. A1293) and NBT/BCIP substrate.
Lanes
A: Antibody dilution 1:8,000
B: Negative control (only secondary antibody)
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MBP-17, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: suitable | |
| western blot: 1:4,000 using purified, recombinant MBP |
| Application: | Endogenous intracellular levels of MBP in salmonella cells were determined by western blot analysis using a monoclonal anti-MBP antibody. |
| Application: | Monoclonal Anti-Maltose Binding Protein antibody produced in mouse has been used in the detection of MBP fusion protein using: • western blotting and in vitro pull-down assay in Arabidopsis thaliana and Oryza sativa proteins • dot blot assay of MBP recombinant G protein of Oryza sativa • C3b complement component binding assay |
| Biochem/physiol Actions: | Maltose Binding Protein (MBP) mediates uptake of maltose. MBP is a substrate for chaperone secB. Fusion of proteins with MBP tag at the N -terminus improves their solubility. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Maltose Binding Protein (MBP) is a periplasmic protein specific to bacteria. It has maltodextrin binding site and binds to linear and cyclic maltodextrins. The mature MBP is synthesized from the pre-protein comprising of 26 amino acids, as signal peptide. |
| General description: | The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins. |
| Immunogen: | Purified, recombinant MBP fusion protein |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |