Methionine Aminopeptidase from Pyrococcus furiosus
SIGMA/M6435 - ≥93% (SDS-PAGE), recombinant, expressed in E. coli
MDL Number: MFCD03457470
Product Type: Chemical
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| assay | ≥93% (SDS-PAGE) |
| foreign activity | Other proteases, none detected |
| form | solution |
| mol wt | 37 kDa by SDS-PAGE |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | 0.5 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P56218  |
| Application: | Methionine Aminopeptidase from Pyrococcus furiosus has been used in a study to analyze the binding of Co(II)-specific inhibitors to the methionyl aminopeptidases from Escherichia coli and Pyrococcus furiosus. It has also been used in a study to examine the binding of a new class of pseudopeptide analog inhibitors. |
| Biochem/physiol Actions: | Thermostable methionine aminopeptidase, which specifically liberates the N-terminal methioinine from proteins and peptides. |
| General description: | Methionine aminopeptidase from Pyrococcus furiosus is a 32 kDa thermostable enzyme. It belongs to type 2a class of methionine aminopeptidase. Methionine aminopeptidase maintains protein homeostasis and coordinates posttranslational modification of proteins in eukaryotes. |
| General description: | X-ray crystallography of the structure of methionine aminopeptidase from Pyrococcus furiosus or PfMAP was performed at a resolution of 1.75A and showed that the protein consists of a catalytic domain containing two cobalt ions in the active site and a unique insertion domain which is specific to the prokaryotic form of the protein. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Other Notes: | One unit will hydrolyze 1 μmol of Met from Met-Pro-Ala-Ala-Gly in 1 minute at pH 7.5 at 37 °C. |
| Physical form: | Solution containing 0.01% Tween® 20, 0.1 mM CoCl2, and 10 mM Tris-HCl, pH 7.5. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥93% (SDS-PAGE) |
| activity | specific activity: 0.5 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.11.18 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |