Anti-Mitofusin 2 antibody produced in rabbit
SIGMA/M6444 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CMT2A; Anti-CMT2A2; Anti-CPRP1; Anti-KIAA0214; Anti-MARF; Anti-Mfn2
MDL Number: MFCD03455396
Product Type: Chemical
Immunoblotting
Rat brain mitochondria (Lane 1) and mouse brain mitochondria (Lane 2) extracts were separated on SDS-PAGE and blotted with 0.25 μg/mL Rabbit Anti-Mitofusin 2 (Cat. No. M6444). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
Rat brain mitochondria (Lane 1) and mouse brain mitochondria (Lane 2) extracts were separated on SDS-PAGE and blotted with 0.25 μg/mL Rabbit Anti-Mitofusin 2 (Cat. No. M6444). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~86 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using HeLa human epitheloid carcinoma cell lysate |
| western blot (chemiluminescent): 0.5-1 μg/mL using extract of mouse and rat brain mitochondria | |
| UniProt accession no. | O95140  |
| Application: | Anti-Mitofusin 2 antibody has been used in immunoblotting. It may also be used in immunoprecipitation. |
| Application: | Anti-Mitofusin 2 antibody produced in rabbit has been used for Western blotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | MFN2 is essential for the maintenance of mitochondrial network and controls mitochondrial metabolism. Mfn2-dependent regulatory mechanism is disturbed in obesity by reduced Mfn2 expression. Mutations in MFN2 cause Charcot Marie-Tooth neuropathy type 2A, a neurological disorder that results from degeneration of axons in peripheral nerves. |
| Biochem/physiol Actions: | Mitofusin-2 (MFN2) plays an important role as a mitochondrial fusion protein and participates in many cell processes. It is mainly involved in regulating metabolic processes in the mitochondria. MFN2 prevents the formation of reactive oxygen species and controls endoplasmic reticulum stress. Mutations in the gene encoding this protein have been linked to diabetes and obesity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Mitofusin 2 is produced in rabbit using as immunogen a synthetic peptide corresponding to human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. Mitofusins (Mfn1 and Mfn2) form homotypic and heterotypic complexes for fusion in mitochondria. |
| General description: | Mitofusin-2 (MFN2) is an outer mitochondrial membrane protein. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 557-576 of human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one and two amino acids in rat and mouse mitofusin 2, respectively. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |