Macrophage Colony-Stimulating Factor human
SIGMA/M6518 - M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: MCSF
MDL Number: MFCD00166591
Product Type: Chemical
Bioassay EC50 is defined as the effective concentration of M-CSF that elicits a 50% attachment factor in cell based bioassay.
SDS-PAGE The purity of recombinant Human M-CSF (Cat. No. M6518) was determined by SDS-PAGE of reduced (+) and non-reduced (-) M-CSF and staining with Coomassie Blue.
| assay | ≥98% (SDS-PAGE and HPLC) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | ≤1.000 EU/μg |
| mol wt | dimer 37 kDa |
| packaging | pkg of 10 μg |
| potency | 0.500-5.000 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P09603  |
| Analysis Note: | The proliferative activity is tested in culture using the cytokine-dependent murine monocytic cell line M-NFS-60 cells. |
| Application: | Macrophage Colony-Stimulating Factor human has been used for the differentiation of bone marrow-derived macrophages (BMDMs) and human monocyte-derived macrophages (MDMs). It has also been used for cell culture. |
| Biochem/physiol Actions: | Macrophage colony-stimulating factor (M-CSF) augments many of the physiological functions of macrophages, and support osteoclast differentiation. |
| General description: | Macrophage Colony-Stimulating Factor (M-CSF) is a hematopoietic growth factor, which modulates the proliferation, differentiation and functional activation of monocytes. It is produced by fibroblasts, endothelial cells and bone marrow stromal cells. MCSF stimulates the functions of mature monocytes and macrophages. |
| Other Notes: | 159 amino acids including N-terminal methionine (each monomer). |
| Physical form: | Lyophilized from a sterile filtered buffered solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE and HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |