Anti-Melan-A antibody, Mouse monoclonal
SIGMA/M6570 - clone A103, purified from hybridoma cell culture
Synonym: Anti-MART1
MDL Number: MFCD01633630
Product Type: Chemical
Immunoblotting Total cell extract of SK Mel19 cells was separated on SDS-PAGE and blotted with Monoclonal Anti-Melan A (Cat. No. M6570). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1: 0.5 μg/mL antibody 2: 0.25 μg/mL antibody 3: 0.125 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | A103, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~22 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 0.1-0.2 μg/mL using total cell extract of SKMEL19 cells () | |
| UniProt accession no. | Q16655  |
| Application: | Anti-Melan-A antibody, Mouse monoclonal has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • immunocytochemistry • immunoprecipitation • immunohistochemistry |
| Biochem/physiol Actions: | Melan-A/MART1 may have a role in pigmentation since its expression pattern and sub cellular distribution is similar to other melanosomal proteins and correlates with melanin content. Melan-A is important for Pmel17 activity, forms a complex with it, and affects its expression, stability, trafficking, and processing that is required for melanosome structure and maturation. Thus, Melan-A is important for Pmel17 function and therefore it is important for mammalian pigmentation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Melan-A is a melanoma antigen recognized by T cells. |
| General description: | Melan-A is also known as MART-1 (melanoma antigen recognized by T-cells 1). It is a transmembrane protein which is hydrophobic in nature. Melan-A is expressed in melanocytes and melanoma tumors. |
| General description: | Monoclonal Anti-Melan-A (mouse IgG1 isotype) is derived from the hybridoma A103 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with a human recombinant Melan-A protein. Melan-A (also known as MART1) is a melanoma antigen recognized by T cells. The protein is expressed mainly in cells and tumors of melanocytic lineage and thus belongs to the melanocyte differentiation markers. |
| Immunogen: | recombinant human Melan-A protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |