Membrane Scaffold Protein 1D1
SIGMA/M6574 - recombinant, expressed in E. coli
Synonym: MSP1D1; MSP1T2
Product Type: Chemical
The concept of membrane scaffold proteins was originally derived from the naturally occurring apoliporotein A-I component of high density lipoprotein. Phospholipid bilayer nanodiscs have been utilized to incorporate a wide variety of anchored and transmembrane proteins. Nanodiscs can provide improved solubility, homogeneity and more native conditions over traditional microsomal preparation.
Fifteen different Membrane Scaffold Protein Constructs are available for applications that require tagging, chemical modifications and acommodations for various size membrane proteins.
The critical component of Nanodiscs is the encircling amphipathic helical protein belt (membrane scaffold protein or MSP). The MSP1D1 construct generates Nanodiscs ~9.8 nm in diameter typically containing ~150 lipid molecules per Nanodisc. The thickness of a Nanodisc can vary depending on the type of phospholipid incorporated (typically 4.6–5.6 nm).
| ε (extinction coefficient) | 18200 M-1cm-1 at 280 nm (His-tag-cleaved dissolved in 20 mM Tris pH 7.4, 0.1M NaCl, 0.5mM EDTA and 0.01%NaN3)(lit.) |
| 21000 M-1cm-1 at 280 nm (uncleaved His-tagged dissolved in 20 mM Tris pH 7.4, 0.1M NaCl, 0.5mM EDTA and 0.01%NaN3)(lit.) | |
| biological source | microbial |
| description | N-Terminal histidine-tagged |
| form | lyophilized powder |
| mol wt | Mw 24661.9 by amino acid sequence |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| Application: | For an extensive list of citations and protocols visit the Sligar Lab Website at; sligarlab.life.uiuc.edu/n |
| Application: | For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page . |
| Application: | Membrane Scaffold Protein 1D1 has been used as a scaffolding protein to stabilize lipid nanodiscs (NDs). It has also been used for the preparation of nanodiscs. |
| Application: | Nanodisc soluble lipid bilayer systems have proven to be a widely applicable means for rendering membrane proteins soluble in aqueous solutions in a native-like bilayer environment where they remain monodisperse and active. The critical component of nanodiscs is the encircling amphipathic helical protein belt (membrane scaffold protein). |
| Application: | The nanodisc system has been employed to incorporate a wide variety of proteins including GPCRs, P450s, bacteriorhodopsin, coagulation factors, cholera toxin, TAR receptor and aromatase. |
| Biochem/physiol Actions: | Generates Nanodiscs ~9.7 nm in diameter |
| Biochem/physiol Actions: | Membrane scaffold protein 1D1 (MSP1D1) is derived from apolipoprotein A-I. It is an amphipathic synthetic protein, which self assembles to form nanodiscs. |
| General description: | Sequence:GHHHHHHHDYDIPTTE |
| Legal Information: | Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois. • 7,691,414 Membrane scaffold proteins • 7,662,410 Membrane scaffold proteins and embedded membrane proteins • 7,622,437 Tissue factor compositions and methods • 7,592,008 Membrane scaffold proteins • 7,575,763 Membrane scaffold proteins and tethered membrane proteins • 7,083,958 Membrane scaffold proteins • 7,048,949 Membrane scaffold proteins |
| Physical form: | Supplied as a lyophilized histidine-tagged protein with a TEV protease cleavage site stabilized with Tris-HCl, EDTA, and NaCl. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P261 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |