Monoclonal Anti-MAT-Tag® antibody produced in mouse
SIGMA/M6693 - purified immunoglobulin, ~2 mg/mL, clone MAT1-87
MDL Number: MFCD06798711
Product Type: Chemical
Western Blotting MAT1-GST purified protein were separated on SDS-PAGE and probed with Monoclonal Anti-MAT-Tag® antibody produced in Mouse, Clone: MAT1-87 (Cat. No. M6693) The anibody was developed with 1:2,000 Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304). Lanes 1. 1.25 μg/mL antibody 2. 2.5 μg/mL antibody 3. 5 μg/mL antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MAT1-87, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg |
| western blot: 1-2 μg/mL |
| Application: | Monoclonal Anti-MAT-Tag® antibody produced in mouse has been used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation • immunocytochemistry |
| Application: | The MAT tag is a seven amino acid peptide tag that binds to transition metals such as Nickel and Cobalt. This tag, which consists of the sequence HNHRHKH, allows the purification of MAT fusion proteins using immobilized metal affinity chromatography (IMAC) such as the highly selective HIS-Select® Nickel Affinity Gel. The MAT tag sequence can be incorporated into both prokaryotic and eukaryotic expression vectors. |
| Biochem/physiol Actions: | Monoclonal Anti-MAT recognizes the seven amino acid MAT epitope sequence within either N or C terminal MAT-tagged fusion proteins. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-MAT (mouse IgG2a isotype) is derived from the MAT1-87 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic MAT peptide HNHRHKHGGGC conjugated to KLH. |
| General description: | Monoclonal Anti-Mat-Tag® antibody was used in one-step purification of histidine-tagged fusion proteins under high pressure and high flow conditions. It is used in the characterization of the molecular architecture of human caveolin-3 and interaction with the skeletal muscle ryanodine receptor. |
| Immunogen: | synthetic MAT peptide HNHRHKHGGGC. |
| Legal Information: | HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | MAT-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied as a solution in 0.01M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |