Anti-MLKL (58-70) antibody produced in rabbit
SIGMA/M6697 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-mixed lineage kinase domain-like (Homo sapiens)
Product Type: Chemical
Immunofluorescence Anti-MLKL (58-70) (RB): Cat. No. M6697: Immunofluorescence of HUVEC cells using MLKL (58-70) (RB), Cat. No. M6697 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
Immunoblotting Lysate of HEK-293T cells overexpressing human MLKL was separated on SDS-PAGE, blotted with Anti-MLKL (58-70) (Cat. No. M6697) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:250
Chart Anti-MLKL (58-70) (RB): Cat. No. M6697: Intensity analysis of Anti-MLKL (58-70) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~54 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:250-1:500 |
| UniProt accession no. | Q8NB16  |
| Application: | Anti-MLKL (58-70) antibody produced in rabbit has been used in western blotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) |
| Application: | western blot: 1:250-1:500 |
| Application: | Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if . |
| Biochem/physiol Actions: | Mixed lineage kinase domain-like protein (MLKL) primarily causes receptor-interacting protein (RIP) kinase-dependent necroptosis. However, during hepatitis, it results in programmed hepatocellular necrosis, which is independent of RIPK3. MLKL also participates in endosomal trafficking and in the formation of extracellular vesicles. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MLKL (mixed lineage kinase domain-like) is a protein that belongs to the protein kinase superfamily. It facilitates necrosis signaling downstream of the kinase RIP3 (receptor-interacting serine-threonine kinase 3) . Anti-MLKL (58-70) antibody can be used in western blotting. Rabbit anti- MLKL (58-70) antibody reacts specifically with MLKL protein. |
| General description: | MLKL is encoded by the gene mapped to human chromosome 16q23. Activated MLKL is localized on the cell membrane. |
| Immunogen: | synthetic peptide corresponding to amino acids 58-70 of human MLKL. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |