Anti-MeCP2 antibody, Mouse monoclonal
SIGMA/M6818 - clone Mec-168, purified from hybridoma cell culture
MDL Number: MFCD02686372
Product Type: Chemical
Immunoblotting
Jurkat cell nuclear extracts were separated on SDS-PAGE and blotted with Monoclonal Anti-MECP2 antibodies. (Lane 1) clone Men-8 (4 μg/mL) (Cat. No. M7443); (Lane 2) clone Mec-168 (0.5 μg/mL) (Cat. No. M6818).
Jurkat cell nuclear extracts were separated on SDS-PAGE and blotted with Monoclonal Anti-MECP2 antibodies. (Lane 1) clone Men-8 (4 μg/mL) (Cat. No. M7443); (Lane 2) clone Mec-168 (0.5 μg/mL) (Cat. No. M6818).
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human Testis sections with 20 μg/mL Monoclonal Anti-MeCP2, Clone: Mec-168 (Cat. No. M6818) using biotin/ExtrAvidin®-Peroxidase.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Mec-168, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~75 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma. | |
| UniProt accession no. | P51608  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunoprecipitation (1 paper) Western Blotting (1 paper) |
| Application: | Monoclonal Anti-MeCP2 antibody produced in mouse has been used in: • immunoblotting • immunoprecipitation • immunostaining • enzyme linked immunosorbent assay (ELISA) • immunocytochemistry • western blot (0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma) |
| Biochem/physiol Actions: | Methyl-CpG binding protein 2 (MeCP2) deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes. Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression. |
| Biochem/physiol Actions: | Methyl-CpG binding protein 2 (MeCP2) is a transcription modulator that binds methylated DNA. This protein regulates neuronal functions and central nervous system development. Alterations in MeCP2 have been associated with neurological diseases such as MECP2 duplication syndrome and Rett syndrome |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Mec-168 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C- terminus of human MeCP2. Methyl-CpG binding protein 2 (MeCP2) is the first methyl-CpG-binding protein to be isolated. This protein contains a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD). |
| Immunogen: | synthetic peptide corresponding to the C-terminus (amino acids 471-486) of human MeCP2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |