Myeloperoxidase from human leukocytes
SIGMA/M6908 - lyophilized powder, ≥50 units/mg protein
Synonym: MPO; Peroxidase, myelo
CAS Number: 9003-99-0
MDL Number: MFCD00131641
Product Type: Chemical
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| biological source | human leucocytes |
| form | lyophilized powder |
| InChI | 1S/H2O3/c1-3-2/h1-2H |
| InChI key | JSPLKZUTYZBBKA-UHFFFAOYSA |
| Quality Level | 200  |
| shipped in | dry ice |
| SMILES string | [O+H2]O[O-] |
| solubility | aqueous buffers such as 50 mM sodium acetate: soluble (Freezing of the enzyme in solution may result in a substantial loss of activity.) |
| H2O: soluble | |
| specific activity | ≥50 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P05164  |
| Analysis Note: | Source material tested and found negative for antibody to HIV and for HBSAG. |
| Application: | Myeloperoxidase (MPO) from human leukocytes has been used in MPO assay. |
| Application: | Myeloperoxidase from human leukocytes has been used: • as a standard to quantify the neutrophil (polymorphonuclear cell [PMN]) infiltration in lung tissue • as a neutrophil extracelullar trap (NET) component to stimulate 106 dendritic cells or 106 macrophages • as a standard in myeloperoxidase (MPO) assay to measure pancreatic MPO |
| Biochem/physiol Actions: | Myeloperoxidase (MPO) has a crucial role to play in destruction of various microorganisms and foreign cells, such as bacteria, fungi, viruses, red cells and malignant and nonmalignant nucleated cells. MPO catalyzes the production of number of reactive oxidant species (ROS) that influence tissue damage during inflammation. MPO and its downstream inflammatory pathways function as a potent therapeutic target for prophylaxis of atherosclerotic cardiovascular disease. MPO catalyze the synthesis of polychlorinated dibenzo(p)dioxins and furans (PCDD/F) from precursor such as chlorophenols in presence of hydrogen peroxide (H2O2). |
| General description: | Myeloperoxidase (MPO) is encoded by the gene mapped to human chromosome 17q22-24. The encoded protein is a leukocyte-derived enzyme and is an integral constituent of the innate immune response. MPO belongs to the heme peroxidase superfamily. MPO is a heme protein containing iron and is highly expressed in leukocytes, neutrophils, monocytes and some subtypes of tissue macrophages. |
| Other Notes: | Lysosomal heme-containing protein. |
| Other Notes: | One unit of myeloperoxidase will catalyze the consumption of one micromole of hydrogen peroxide and the production of 1/4 micromole of tetraguaiacol per minute at pH 7.0 and 25 °C |
| Physical form: | Lyophilized from 50 mM sodium acetate buffer, pH 6.0, 0.1 M sodium chloride |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.11.2.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352200 |