M2 medium
SIGMA/M7167 - With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture
Synonym: Cell Culture M2; Cell Culture Medium; M2 Medium Solution
MDL Number: MFCD00283761
Product Type: Chemical
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| components | HEPES: 5.42726 g/L NaHCO3: 0.35 g/L glucose: 1.0 g/L (Dextro) phenol red: 0.0106 g/L sodium pyruvate: 0.0363 g/L |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 300  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage condition | protect from light |
| storage temp. | 2-8°C |
| technique(s) | cell culture | embryo: suitable |
| Analysis Note: | Presence of particulates in solution will not affect product′s performance. |
| Analysis Note: | This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst. |
| Application: | Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently. M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator. |
| Preparation Note: | Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |