α-Mannosidase from Canavalia ensiformis (Jack bean)
SIGMA/M7257 - ammonium sulfate suspension, ≥15 units/mg protein (biuret)
Synonym: α-D-Mannoside mannohydrolase
CAS Number: 9025-42-7
EC Number: 232-794-1
MDL Number: MFCD00130495
Product Type: Chemical
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| biological source | Canavalia ensiformis |
| foreign activity | β-galactosidase, β-N-acetylglucosaminidase |
| form | ammonium sulfate suspension |
| InChI | 1S/Zn/q+2 |
| InChI key | PTFCDOFLOPIGGS-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | [Zn+2] |
| specific activity | ≥15 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| Application: | Liberates mannose from a variety of synthetic and natural α-mannosides. |
| Application: | α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice. |
| Application: | α-Mannosidase from Canavalia ensiformis (Jack bean) has been used: • for the digestion of neutral glycans. • to remove O-glycans from recombinant β-glucosidase • for the inhibition of renal fibroblasts proliferation |
| Biochem/physiol Actions: | α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins. |
| Biochem/physiol Actions: | α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues. |
| Biochem/physiol Actions: | α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes. |
| General description: | Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins. |
| General description: | α-Mannosidase is an exo-glycosidase and an inc metalloenzyme. |
| General description: | α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl α- |
| Physical form: | Suspension in 3.0 M (NH4)2SO4 and 0.1 mM zinc acetate, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥15 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.24 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |