N,N′-Methylenebisacrylamide
SIGMA/M7279 - powder, Molecular Biology, suitable for electrophoresis, ≥99.5%
Synonym: BIS
CAS Number: 110-26-9
Empirical Formula (Hill Notation): C7H10N2O2
Molecular Weight: 154.17
EC Number: 203-750-9
MDL Number: MFCD00008625
Linear Formula: (CH2=CHCONH)2CH2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M7279-25G
This image depicts SKU: M7279-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M7279-100G
This image depicts SKU: M7279-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M7279-250G
This image depicts SKU: M7279-250G
| absorption | ≤0.2 at 290 at 1% |
| anion traces | chloride (Cl-): ≤50 mg/kg |
| assay | ≥99.5% |
| cation traces | Ca: ≤10 ppm |
| Cd: ≤5 ppm | |
| Co: ≤5 ppm | |
| Cr: ≤5 ppm | |
| Cu: ≤5 ppm | |
| Fe: ≤5 ppm | |
| K: ≤50 ppm | |
| Mg: ≤5 ppm | |
| Mn: ≤5 ppm | |
| Na: ≤50 ppm | |
| Ni: ≤5 ppm | |
| Pb: ≤5 ppm | |
| Zn: ≤5 ppm | |
| conductivity | ≤10 μmho (2% solution) |
| foreign activity | DNase and RNase, none detected |
| form | powder |
| grade | Molecular Biology |
| impurities | ≤0.05% Free carboxylic acid (T) |
| ≤50 mg/kg Total sulfur (as SO4 ) (ICP) | |
| InChI | 1S/C7H10N2O2/c1-3-6(10)8- |
| InChI key | ZIUHHBKFKCYYJD-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | C=CC(=O)NCNC(=O)C=C |
| technique(s) | electrophoresis: suitable |
| Application: | N,N′-Methylenebisacrylamide has been used: • as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix • as a crosslinker to prepare tough hydrogel network • in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel |
| Application: | To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis. |
| General description: | N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa. |
| Packaging: | 25, 100, 250 g in poly bottle |
| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H301 - H340 |
| Precautionary statements | P202 - P264 - P270 - P280 - P301 + P310 - P405 |
| Hazard Codes | Xn |
| Risk Statements | 20/22 |
| Safety Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Purity | ≥99.5% |
| UNSPSC | 41105319 |