M16 Medium
SIGMA/M7292 - With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
Synonym: Cell Culture Medium; Cell Culture Medium M16; M16 Medium Solution
Product Type: Chemical
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| components | NaHCO3: 2.101 g/L phenol red: 0.0106 g/L sodium pyruvate: 0.0363 g/L glucose: 1.0 g/L (Dextro) |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 300  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | embryo: suitable |
| Analysis Note: | Presence of particulates in solution will not affect product′s performance. |
| Analysis Note: | This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst. |
| Application: | M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently. |
| Preparation Note: | Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |