Anti-MBD2a,b (RA-18) antibody produced in rabbit
SIGMA/M7318 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DMTase; Anti-NY-CO-41
MDL Number: MFCD06798469
Product Type: Chemical
Immunofluorescence
Nuclear localization of MBD2a,b in NIH-3T3 cells. Cells were stained with Rabbit Anti-MBD2a,b (RA-18) (Cat. No. M7318). 40X magnification (A); 100X magnification (B).
Nuclear localization of MBD2a,b in NIH-3T3 cells. Cells were stained with Rabbit Anti-MBD2a,b (RA-18) (Cat. No. M7318). 40X magnification (A); 100X magnification (B).
Immunoblotting
Nuclear extracts of MCF7 cells (Lanes 1, 3) and HeLa cells (Lanes 2, 4) were separated on SDS-PAGE, blotted with Rabbit Anti-MBD2a,b (RA-18) (Cat. No. M7318) and developed using Goat anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. (Lane 1) Antibody 0.5 μg/mL; (Lane 2) Antibody 1 μg/mL; (Lane 3) Antibody 0.5 μg/mL + 10 μg/mL MBD2a,b immunizing peptide; (Lane 4) Antibody 1 μg/mL + 10 μg/mL MBD2a,b immunizing peptide.
Nuclear extracts of MCF7 cells (Lanes 1, 3) and HeLa cells (Lanes 2, 4) were separated on SDS-PAGE, blotted with Rabbit Anti-MBD2a,b (RA-18) (Cat. No. M7318) and developed using Goat anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. (Lane 1) Antibody 0.5 μg/mL; (Lane 2) Antibody 1 μg/mL; (Lane 3) Antibody 0.5 μg/mL + 10 μg/mL MBD2a,b immunizing peptide; (Lane 4) Antibody 1 μg/mL + 10 μg/mL MBD2a,b immunizing peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells |
| microarray: suitable | |
| western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells | |
| UniProt accession no. | Q9UBB5  |
| Application: | Anti-MBD2a,b (RA-18) antibody produced in rabbit has been used: • for chromatin immunoprecipitation and western blotting • immunofluorescence • immunoblotting |
| Biochem/physiol Actions: | MBD2 binds to methylated DNA in vitro or in vivo, suggesting that MBD2 targets the MeCP1 complex to methylated DNA. MBD2a interacts with RNA Helicase A (RHA). MBD2 protein appear to protect against some cancers. |
| Biochem/physiol Actions: | Methyl-CpG binding domain protein 2 (MBD2a) acts as a repressor during transcription. |
| Biochem/physiol Actions: | The antibody recognizes MBD2a (~45 kDa) and MBD2b (~29 kDa). In immunoblotting, an unidentified band at ~43 kDa may also be detected. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-MBD2a,b (RA-18) is developed in rabbit using as immunogen a synthetic peptide corresponding human MBD2a conjugated to KLH. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene; MBD2a is a 414 amino acids protein, whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a is a component of the MeCP1 corepressor complex, which is a 400-800 kDa complex containing as components MBD2, Mi-2, MTA2, MBD3, and HDAC1/2. |
| General description: | The gene encoding methyl-CpG binding domain protein 2 (MBD2a) is localized on chromosome 18. |
| Immunogen: | synthetic peptide corresponding to amino acids 294-411 of human MBD2a conjugated to KLH via an N-terminal added lysine residue. This sequence is conserved in MBD2a and MBD2b of human origin, and is not found in MBD3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |