Anti-MeCP2 antibody, Mouse monoclonal
SIGMA/M7443 - clone Men-8, purified from hybridoma cell culture
Synonym: Anti-AUTSX3; Anti-MRX16; Anti-MRX79; Anti-MRXS13; Anti-MRXSL; Anti-PPMX; Anti-RS; Anti-RTS; Anti-RTT
MDL Number: MFCD02686372
Product Type: Chemical
Immunoblotting
Jurkat cell nuclear extracts were separated on SDS-PAGE and blotted with Monoclonal Anti-MECP2 antibodies. (Lane 1) clone Men-8 (4 μg/mL) (Cat. No. M7443); (Lane 2) clone Mec-168 (0.5 μg/mL) (Cat. No. M6818).
Jurkat cell nuclear extracts were separated on SDS-PAGE and blotted with Monoclonal Anti-MECP2 antibodies. (Lane 1) clone Men-8 (4 μg/mL) (Cat. No. M7443); (Lane 2) clone Mec-168 (0.5 μg/mL) (Cat. No. M6818).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Men-8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~75 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/test using nuclear extract of cultured human acute T cell leukemia Jurkat cells or MCF7 cells | |
| UniProt accession no. | P51608  |
| Application: | Monoclonal Anti-MeCP2 antibody has been used: • in western blotting • in immunoblotting • in flow cytometry • in immunohistofluorescence • in immunocytochemistry |
| Biochem/physiol Actions: | MeCP2 (methyl-CpG binding protein 2) silences transcription by recruiting the histone deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes. |
| Biochem/physiol Actions: | Methyl-CpG binding protein 2 (MeCP2) has been shown be crucial for brain development. It part of the methyl-CpG-binding proteins, which are involved in repression of gene expression by binding to methylated DNA. Mutations in the gene encoding MeCP2 have been associated with Rett syndrome (RTT). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Methyl-CpG binding protein 2 (MeCP2) possesses a methyl-CpG-binding domain (MBD) and a transcriptional repression domain. |
| General description: | Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Men-8 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminus of human MeCP2. |
| Immunogen: | synthetic peptide corresponding to the N-terminus (amino acids 15-30) of human MeCP2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |