MG-132, Ready Made Solution

SIGMA/M7449 - ≥90% (HPLC)

Synonym: MG-132 Proteasome Inhibitor

EC Number: 200-664-3
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-M7449-200UL	200 µL	$102.00 1/EA		Add To Favorites
45-M7449-1ML	1 mL	$335.00 1/EA		Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

assay	≥90% (HPLC)
concentration	10 mM in DMSO
form	DMSO solution
quality	useful for proteasome inhibition
Quality Level	200
shipped in	dry ice
storage temp.	−20°C

Amino Acid Sequence:	Z-Leu-Leu-Leu-al
Application:	MG-132, Ready Made Solution has been used to inhibit the proteasome in various systems, including: • hTERT-RPE1 and HeLa cells: Osorio-Zambrano, W.F. et al., PLoS One, 10(12), e0144434 (2015) • FaDu human hypopharynx cancer cells: Kim, S.-Y. et al., Autophagy, 14(3), 385-403 (2018) • HEK293 cells: Chen, Q. et al., Cell Reports, 22(11), 2860-2872 (2018). • Neonate rat cardiomyocytes: Turkieh, A. et al., Cell Death Dis., 10(8), 608 (2019) • Adult neural stem cells: Morrow, C.S. et al., Cell Stem Cell, 26(4), 558-568.e9 (2020).
Biochem/physiol Actions:	MG132, also known as carbobenzoxy-Leu-Leu-leucinal, is a peptide aldehyde which plays a vital role in the inhibition of proteolytic activity of the 26S proteasome complex. MG132 retards the growth of HeLa cells by promoting cell cycle arrest and apoptosis. Treatment of HepG2 and HeLa cells with MG-132 triggers increased expression of MCPIP1 mRNA, which in turn increases MCPIP1 protein concentration. MCPIP1 is implicated in negative regulation of macrophage activation and differentiation of several cell types, including pre-adipocytes, neuroprogenitor cells and osteoclast precursors. MG-132 stimulates dopamine reduction and nigral dopaminergic degeneration in both cell culture and animal models of Parkinson’s disease.
Biochem/physiol Actions:	Potent, membrane-permeable proteasome inhibitor. Induces neurite outgrowth in PC12 cells at 10 μM. Blocks cleavage of poly(ADP-ribose) polymerase and apoptosis in thymocytes. Proteasome inhibition induces accumulation of heat shock protein mRNA, activation of heat-shock proteins, and enhanced thermotolerance in various cell types: however, it also activates JNK-1, which initiates apoptosis in response to cell stress.
Features and Benefits:	• Ready-to-use solution in DMSO • Purity tested by HPLC
General description:	MG132 is a potent proteasome inhibitor that also inhibits calpains and lysosomal cathepsins. One of the first proteasome inhibitors to be developed, MG-132 is widely used in proteasome inhibition studies because of its high potency, high selectivity, and rapid reversibility. MG132 mechanistically acts as a proteasome inhibitor by hemiacetal formation with hydroxyl groups of active site threnonine residues.
Physical form:	MG-132, Ready Made Solution is supplied as a 10 mM (1 mg/210 μL) 0.2 μm-filtered solution in DMSO.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	188.6 °F - closed cup
Flash Point(C)	87 °C - closed cup

Purity	≥90% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352200

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