Anti-Myosin (Skeletal) antibody produced in rabbit
SIGMA/M7523 - whole antiserum
MDL Number: MFCD00162704
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tongue sections were stained with 1:20 Anti-Myosin (Skeletal) antibody produced in Rabbit (Cat. No. M7523) followed by 1:20 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Pig tongue sections stained with 1:40 Anti-Myosin (Skeletal) antibody produced in Rabbit (Cat. No. M7523) (A). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887), and 1:20 Anti-Myosin (Skeletal, Fast) antibody, Mouse monoclonal, Clone: DE-U-10 (Cat. No. SAB4200707) (B). The antibody was developed using Rabbit Anti-Mouse IgG-Cy3 conjugate antibody. Results Two Anti-Myosin antibodies, D8281 (A) and SAB4200707 (B), target different regions of Myosin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human or animal skeletal muscle |
| indirect immunofluorescence: 1:20 using human or animal sletetal muscle | |
| UniProt accession no. | P11055  |
| Analysis Note: | The antiserum has been treated to remove lipoproteins. |
| Application: | Anti-Myosin (Skeletal) antibody produced in rabbit has been used in: • immunohistochemistry • immunostaining • western blotting • immunofluorescence • immunolocalization • immunocytochemistry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | Specifically labels the A bands of human and animal skeletal muscle. Does not stain human smooth muscle tissue. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myosin is a 480,000 dalton protein containing two identical heavy chains (200,000 daltons each) and four light chains (15,000-26,000 daltons). Myosin molecules consist of two major regions: tails (rods) and heads; they aggregate into filaments through the tail region. Multiple forms of myosin heavy chains exist for each muscle type; skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. |
| Immunogen: | whole myosin (heavy and light chains) from human skeletal muscle. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |