Medium 199
SIGMA/M7528 - HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym: Cell culture medium; M199 Medium; TCM 199
Product Type: Chemical
Medium 199 This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
| components | L-glutamine: no sodium pyruvate: no HEPES: 25 mM phenol red: yes NaHCO3: yes Earle’s salts (5% CO2): yes |
| form | liquid |
| impurities | endotoxin, tested |
| pH | >7.2 |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| General description: | Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues. |
| Preparation Note: | Supplement with 0.1 g/L |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |