Anti-Myosin (Skeletal and Smooth) antibody produced in rabbit
SIGMA/M7648 - whole antiserum
MDL Number: MFCD00162703
Product Type: Chemical
Immunofluorescence NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:10 Anti-Myosin (Skeletal and Smooth) antibody produced in Rabbit (Cat. No. M7648) followed by 1:16 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | animal, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:10 using cultured fibroblasts |
| UniProt accession no. | P35579  |
| P35580 |
| Application: | Anti-Myosin (Skeletal and Smooth) antibody produced in rabbit has been used in indirect immunofluorescence labeling. |
| Biochem/physiol Actions: | Myosin plays a vital role in neurosensory and cortical function, membrane vesicle trafficking and determinant partitioning. Myosins with actin-stimulated ATPase activity is essential for various cellular movements. Myosins I and V mediates vesicle translocation. Myosin II with a long rod like tail domain, assembles into a bipolar thick filament and facilitate muscle contraction. |
| Biochem/physiol Actions: | Shows a pattern of staining along stress fibers of cultured human and animal fibroblasts. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myosin is a 500 kD protein that interacts with actin in muscle and in non-muscle cells. It is characterized with two identical heavy chains (200 kD each) and four light chains (15-20 kD each). Myosin molecules consist of two major regions; the tail (rod) and head. These molecules aggregate into filaments through the tail region and interact with actin and ATP through the head region. |
| Immunogen: | whole myosin (heavy and light chains) from bovine uterus. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |