Anti-MDM2 antibody ,Mouse monoclonal
SIGMA/M7815 - clone HDM2-323, purified from hybridoma cell culture
Synonym: Anti-ACTFS; Anti-HDMX; Anti-LSKB; Anti-hdm2
Product Type: Chemical
Immunoblotting Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-MDM2 Clone: HDM2-323 (Cat. No. M7815). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HDM2-323, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~90 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| microarray: suitable | |
| western blot: 1-2 μg/mL (whole cell extract of 293T cells transfected with human MDM2) | |
| UniProt accession no. | Q00987  |
| Application: | Anti-MDM2 antibody, Mouse monoclonal has been used in western blotting and enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | MDM2 functions in the activation of p53 resulting in MDM2 expression, which consequently inhibits p53 transcriptional function. MDM2 accomplishes this inhibition in two ways: because of its physical interaction with p53, MDM2 both represses p53 transcriptional activity and mediates the degradation of p53. Overexpression of MDM2 results in reduced quantities of coexpressed p53, and disruption of the p53-MDM2 interaction by mutation results in both activation and accumulation of p53. MDM2 protein has been shown to activate cell proliferation by stimulating the S-phase-inducing transcription factors, E2F1/DP1.5 The amplification of the MDM2 gene has been demonstrated in various human neoplasms. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-MDM2 (mouse IgG2a isotype) is derived from the HDM2-323 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. MDM2 (the human homologue of Mdm2, a 90 kDa molecule), is a transcriptional target of p53. |
| Immunogen: | synthetic peptide corresponding to amino acids 389-402 of human MDM2 conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |