Anti-MAGE-C1 antibody, Mouse monoclonal
SIGMA/M7820 - clone CT7-33, purified from hybridoma cell culture
Synonym: Anti-CT7
Product Type: Chemical
Western Blotting Whole extract of SKML37 cells were separated on SDS-PAGE and probed with Anti-MAGE-C1 antibody, Mouse monoclonal, Clone: CT7-33 (Cat. No. M7820). The antibody was developed using Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CT7-33, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~180 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-2 μg/mL using total cell extract of SKML37 cells | |
| UniProt accession no. | O60732  |
| Application: | • Anti-MAGE-C1 antibody, Mouse monoclonal has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunocytochemistry • immunoblotting • immunoprecipitation • immunohistochemistry |
| Biochem/physiol Actions: | Although the physiological role of melanoma-associated antigen (MAGE) proteins is unknown, it has been reported that MAGE-C1 interacts and co-localizes with an additional cancer testis antigen, NY-ESO-1, in melanoma cells. |
| Biochem/physiol Actions: | MAGE family member C1 (MAGE-C1) has been studied as the most expressed cancer or testis antigen in multiple myeloma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-MAGE-C1 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CT7-33 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with human recombinant MAGE-C1. The first melanoma-associated antigen (MAGE) to be identified was MAGE-A1. The MAGE family of proteins contains over 25 genes that have now been identified in human. These proteins share a homologous region, named the MAGE homology domain (MHD). |
| General description: | MAGE family member C1 (MAGE-C1) belongs to the melanoma antigen gene (MAGE) family and consists of 1142 amino acids. The gene encoding MAGE-C1 is localized on human chromosome Xq26-27 and has four exons. |
| General description: | MAGE-C1 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. |
| Immunogen: | human recombinant MAGE-C1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |