Monoclonal Anti-Myosin Light Chain Kinase antibody produced in mouse
SIGMA/M7905 - clone K36, ascites fluid
Synonym: Anti-KRP; Anti-MLCK; Anti-MLCK1; Anti-MLCK108; Anti-MLCK210; Anti-MMIHS; Anti-MMIHS1; Anti-MSTP083; Anti-MYLK1; Anti-smMLCK
MDL Number: MFCD00164705
Product Type: Chemical
Western Blotting Whole extract of Chicken gizzard was separated on SDS-PAGE and probed with 1:10,000 Monoclonal Anti-Myosin Light Chain Kinase antibody produced in Mouse clone K36 (Cat. No. M7905). The antibody was developed using Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917) and chemiluminescent substrate. Lanes 1. 1:10,000 antibody 2. 1:20,000 antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | K36, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | avian, mammals |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| microarray: suitable | |
| western blot: 1:10,000 |
| Application: | Monoclonal anti-myosin light chain kinase antibody has been used in: • immunohistochemistry • immunoblot (diluted 1:10,000) analysis • flow cytometry |
| Biochem/physiol Actions: | In immunoblotting, the antibody recognizes the myosin light chain kinase of smooth muscle from various sources including chicken gizzard, turkey gizzard, and pig stomach as well as the myosin light chain kinase of non-muscle cells such as cultured fibroblasts. |
| Biochem/physiol Actions: | Myosin Light Chain Kinase (MLCK) has a pivotal role in phosphorylating myosin regulatory light chains and hence facilitates its interaction with actin filaments for smooth muscle contraction. Monoclonal anti-myosin light chain kinase antibody can be used for studying the function of the enzyme and its interactions with other cell components. It can also be used in microarray and immunoprecipitation. Mouse anti-myosin light chain kinase antibody reacts specifically with myosin light chain kinase present in smooth muscle of chicken gizzard, turkey gizzard, and pig stomach. This product also shows reactivity for myosin light chain kinase of non-muscle cells like cultured fibroblasts. |
| Biochem/physiol Actions: | The catalytic subunit of cardiac and skeletal muscle myosin light chain kinase is in the range of 80-95 kDa. Proteolysis of the myosin light chain kinase from chicken gizzard produces a 64 kDa fragment that neither binds to Ca2+/calmodulin nor exhibits catalytic activity and a 61 kDa peptide that is active in the absence of Ca2+/calmodulin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Myosin Light Chain Kinase (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin light chain kinase (MLCK) is a calcium/calmodulin-depend |
| Immunogen: | chicken gizzard myosin light chain kinase. |
| Physical form: | The product is provided as ascites fluid with 0.1% sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |