Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit
SIGMA/M7927 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD01633615
Product Type: Chemical
Western Blotting Whole extract of NIH-3T3 Mouse fibroblasts cells were separated on SDS-PAGE and probed with Anti-MAP Kinase (ERK-1, 351-368) antibody produced in Rabbit (Cat. No. M7927). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:10,000 antibody 2. 1:5,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen, ERK-1 44 kDa |
| antigen, ERK-2 42 kDa | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5 μg using NIH 3T3 mouse fibroblast lysates |
| microarray: suitable | |
| western blot: 1:5,000 using NIH 3T3 mouse fibroblast lysate or rat brain extract | |
| UniProt accession no. | P27361  |
| Application: | Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit has been used in: • immunofluorescence • immunoprecipitation • western blotting. |
| Biochem/physiol Actions: | By immunoblotting, the antibody reacts with ERK-1 and ERK-2. |
| Biochem/physiol Actions: | Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), c-Jun N-terminal kinases (JNK) and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK1 (p44) and ERK2 (p42) require the dual phosphorylation in the catalytic kinase domain by MEKs for their full activity. ERK1 is phosphorylated on Tyr204 and Thr202, and ERK2 on Tyr187 and Thr185. ERK1 and 2 may also undergo autophosphorylation on these residues. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mitogen-activated protein kinase 3 (ERK1) is mapped to human chromosome 16p11.2. It has a serine/threonine domain and a transactivation domain (TAD) and a nuclear localization sequence (NLS). |
| Immunogen: | synthetic peptide corresponding to amino acids 351-368 of human ERK-1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |