Anti-Myosin IIB, non-muscle antibody produced in rabbit
SIGMA/M7939 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Myosin-10
MDL Number: MFCD03455611
Product Type: Chemical
Western Blotting Whole extract of cultured Human Jurkat cells were separated on SDS-PAGE and probed with Anti-Myosin IIB, non-muscle antibody produced in Rabbit (Cat. No. M7939). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:200 antibody 2. 1:400 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~200 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot (chemiluminescent): 1:200 using whole cell extracts of cultured dog MDCK kidney cells, cultured rat NRK cells, and cultured human Jurkat cells. |
| UniProt accession no. | P35580  |
| Application: | Anti-Myosin IIB, non-muscle antibody produced in rabbit has been used in: • immunoblotting • stimulated emission depletion (STED) nanoscopy • immunofluorescence |
| Biochem/physiol Actions: | Anti-Myosin IIB, non-muscle antibody is specific for the heavy chain of human myosin IIB (nonmuscle). The antibody also reacts with myosin in dogs and rats. |
| Biochem/physiol Actions: | Myosin IIB plays a key role in the COS-7 cell cytokinesis and is need for the initiation of ring constriction. |
| Biochem/physiol Actions: | Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin IIb regulates memory and synaptic plasticity functions. Nonmuscle myosin IIb has been implicated in fibroblast movement and erythroblast enucleation . |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myosin IIB (nonmuscle) is a relatively abundant, widespread two headed myosin composed of an N-terminal motor domain, a light chain binding neck region, a coiled-coil region, and a nonhelical C-terminal domain. It forms a heterohexamer composed of a pair of heavy chains and two pairs of light chains. Myosin IIB is expressed in embryonic cardiac myocytes. |
| Immunogen: | Synthetic peptide corresponding to amino acid residues 1965-1976 of the heavy chain of human myosin IIB (nonmuscle) conjugated to KLH. This sequence is identical in rabbit and differs by one and two amino acids from the respective bovine and rat sequences. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |