Anti-Myosin IIA, non muscle antibody produced in rabbit
SIGMA/M8064 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-BDPLT6; Anti-DFNA17; Anti-EPSTS; Anti-FTNS; Anti-MATINS; Anti-MHA; Anti-NMHC-II-A; Anti-NMMHC-IIA; Anti-NMMHCA
MDL Number: MFCD03455610
Product Type: Chemical
Immunofluorescence NRK Rat kidney cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with1:100 Anti-Myosin IIA, non-muscle antibody produced in Rabbit (Cat. No. M8064). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of MDCK cells were separated on SDS-PAGE and probed with Anti-Myosin IIA, non muscle antibody produced in Rabbit (Cat. No. M8064). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. 1:1,000 antibody + inhibitor 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~200 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:100 using cultured rat NRK cells |
| microarray: suitable | |
| western blot: 1:1,000 using whole cell extracts of cultured dog MDCK kidney cells and cultured human Jurkat cells | |
| UniProt accession no. | P35579  |
| Application: | Anti-Myosin IIA, non muscle antibody produced in rabbit has been used in: • immunofluorescence • immunohistochemistry • fixed cell staining/ immunofluorescence staining • immunoblot • immunoprecipitation |
| Biochem/physiol Actions: | Nonmuscle myosin II is involved in cell motility and adhesion, cytokinesis, vesicular transport, intracellular force generation and in morphogenesis during development. Its activity is regulated by light chain and possibly heavy chain phosphorylation and by association with proteins such as Mts1. Mutations in the NMHCA gene are found in several syndromes associated with megakaryocyte/platelet/le |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Myosin heavy chain 9 (MYH9) gene codes for the nonmuscle myosin heavy chain IIA. |
| General description: | Myosin heavy chain 9 is expressed in platelets and the gene encoding it is localized on chromosome 22. |
| Immunogen: | synthetic peptide corresponding to amino acids 1949-1960 of human nonmuscle myosin IIA. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |