Monoclonal Anti-Mad1 antibody produced in mouse
SIGMA/M8069 - ~2 mg/mL, clone 9B10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Mitotic Arrest Deficient
MDL Number: MFCD08705588
Product Type: Chemical
Western Blotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Anti-Mad1 antibody, Mouse monoclonal (Cat. No. M8069). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. 1:4,000 antibody 4. Negative control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 9B10, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~85 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 10-20 μg/mL using HeLa cells |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| UniProt accession no. | Q9Y6D9  |
| Application: | Monoclonal Anti-Mad1 antibody produced in mouse has been used in • immunoblotting • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | Mitotic arrest deficient-like 1 (MAD1) functions as a regulator in the segregation of chromosomes during mitosis. It recruits mitotic arrest deficient-like 2 (MAD2) to kinetochores and directs it to function as an inhibitor. MAD1 also helps MAD2 to associate with translocated promoter region (TPr). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mitotic arrest deficient-like 1 (MAD1) is part of the cell-cycle checkpoint. |
| General description: | Monoclonal Anti-Mad1 (mouse IgG2b isotype) is derived from the hybridoma 9B10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with human recombinant Mad1 protein. |
| Immunogen: | recombinant human Mad1 protein. |
| Physical form: | Solution in 0.01 M phosphae buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |