Anti-MAFF (C-Terminal) antibody produced in rabbit
SIGMA/M8194 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-v-maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Family, ProteinF
MDL Number: MFCD08705477
Product Type: Chemical
Immunoprecipitation
(Lanes 2, 3, 4) 2, 5, and 10 μL of Rabbit Anti-MAFF (C-terminal) (Cat. No. M8194) respectively were used to immunoprecipitate human MAFF from 0.5 mg of A431 cell lysate. Detection antibody: Rabbit Anti-MAFF (N-terminal) (Cat. No. M8569). MAFF immunoprecipitates at ~18 kDa. (Lane 1) Negative control — without cell lysate; (Lane 5) Negative control — without IP antibody.
(Lanes 2, 3, 4) 2, 5, and 10 μL of Rabbit Anti-MAFF (C-terminal) (Cat. No. M8194) respectively were used to immunoprecipitate human MAFF from 0.5 mg of A431 cell lysate. Detection antibody: Rabbit Anti-MAFF (N-terminal) (Cat. No. M8569). MAFF immunoprecipitates at ~18 kDa. (Lane 1) Negative control — without cell lysate; (Lane 5) Negative control — without IP antibody.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 18 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-4 μL using A431 cell line |
| microarray: suitable | |
| western blot: 1:2,000-1:4,000 using whole extracts of the A431 cell line | |
| UniProt accession no. | Q9ULX9  |
| Application: | Anti-MAFF (c-terminal) antibody produced in rabbit has been used in: • western blotting • chromatin immunoprecipitation (chip) • co-immunoprecipitation • immunoprecipitation |
| Biochem/physiol Actions: | Anti-MAFF (C-terminal) specifically recognizes MAFF. |
| Biochem/physiol Actions: | The small MAF proteins are associated with many physiological processes including development, differentiation, hematopoiesis and stress response. Interestingly, these proteins regulate the stress response via different mechanisms. The MAFF gene is down-regulated in diabetes mellitus 1 patient. This gene is underexpressed in acute lymphoblastic lymphoma (ALL) patients. |
| Biochem/physiol Actions: | v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) has been shown to interact with other transcription factors and it associates with certain regulatory sequences in the gene encoding the oxytocin receptor. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The MAFF gene is located on the human chromosome at 22q13.1. The encoded protein is a small MAF protein and is expressed in the nucleus. |
| General description: | v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) is a transcription factor which does not have a transactivation domain but possesses a leucine-zipper. |
| Immunogen: | synthetic peptide corresponding to amino acids 147-163 of human MAFF (isoform a). The immunizing peptide does not cross-react with other members of the family. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |