MES hydrate
SIGMA/M8250 - ≥99.5% (titration)
Synonym: 2-
CAS Number: 1266615-59-1
Empirical Formula (Hill Notation): C6H13NO4S · xH2O
Molecular Weight: 195.24 (anhydrous basis)
MDL Number: MFCD00149409
Linear Formula: C6H13NO4S · xH2O
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M8250-100G
This image depicts SKU: M8250-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M8250-25G
This image depicts SKU: M8250-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M8250-250G
This image depicts SKU: M8250-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: M8250-1KG
This image depicts SKU: M8250-1KG
| application(s) | agriculture clinical research diagnostic assay manufacturing life science and biopharma |
| assay | ≥99.5% (titration) |
| biological source | (Chemical/Synthestic) |
| color | white |
| form | crystalline powder |
| impurities | ≤5 ppm (Total: Ag, As, Bi, Cd, Cu, Hg, Mo, Pb, Sb, Sn) |
| InChI | 1S/C6H13NO4S.H2O/c8-12(9, |
| InChI key | MIIIXQJBDGSIKL-UHFFFAOYSA |
| pKa | 6.1 |
| Quality Level | 400  |
| SMILES string | O.OS(=O)(=O)CCN1CCOCC1 |
| solubility | water: 0.25 g/mL, clear, colorless |
| storage condition | dry at room temperature |
| storage temp. | room temp |
| suitability | suitable for electrophoresis |
| technique(s) | cell culture | plant: suitable |
| immunofluorescence: suitable | |
| useful pH range | 5.5-6.7 |
| Application: | MES hydrate has been used:• in the MS (Murashige and Skoog) media for growth of Arabidopsis seedlings. • in the cytoskeleton buffer during immunofluorescence study. • as a wash buffer to wash the paramagnetic beads and resuspend them for binding mannose-BSA • as a component of growth medium |
| Features and Benefits: | • Effective Buffering from pH 5.5-6.7 with a pKa of 6.1 (25 °C) • Highly soluble in water • Minimal metal ion binding • Stable in a wide pH range • Low UV absorptivity and Minimal reactivity |
| General description: | MES hydrate, or 4-morpholineethanesulfoni With excellent water solubility and virtual insolubility in lipids, MES Buffer is impermeable to membranes, ensuring efficacy in cellular environments. Its applications span maintaining stability in cell culture media, protein-based buffer formulations, and electrophoresis running buffers. MES Buffer may also find usage in purifying antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as a better alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers such as citrate and malate. MES Buffer proves valuable for adjusting growth media pH, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 25, 100, 250 g in poly bottle |
| Packaging: | 25, 50 kg in poly drum |
| Preparation Note: | A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered. |
| Preparation Note: | Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.5% (titration) |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |