Anti-AtMPK3 antibody produced in rabbit
SIGMA/M8318 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Arabidopsis thaliana MAP Kinase 3
MDL Number: MFCD07370259
Product Type: Chemical
Immunoblotting
Arabidopsis thaliana whole leaf extracts (Lane A) and seedlings (Lane B), and tobacco whole leaf extract (Lanes C, D) were separated on SDS-PAGE, blotted with Rabbit Anti-AtMPK3 (Cat. No. M8318) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Antibody concentration 1.0 μg/mL (Lanes A, B, C, E), 0.2 μg/mL (Lane D). Inhibition of antibody staining of Arabidopsis thaliana whole leaf extract with the AtMPK3 immunizing peptide (20 μg/mL, Lane E).
Arabidopsis thaliana whole leaf extracts (Lane A) and seedlings (Lane B), and tobacco whole leaf extract (Lanes C, D) were separated on SDS-PAGE, blotted with Rabbit Anti-AtMPK3 (Cat. No. M8318) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Antibody concentration 1.0 μg/mL (Lanes A, B, C, E), 0.2 μg/mL (Lane D). Inhibition of antibody staining of Arabidopsis thaliana whole leaf extract with the AtMPK3 immunizing peptide (20 μg/mL, Lane E).
Immunoblotting
Arabidopsis thaliana whole leaf extract was separated by SDS-PAGE and blotted with: (Lane A) Rabbit Anti-AtMPK3 (0.5 μg/mL) (Cat. No. M8318); (Lane B) Rabbit Anti-AtMPK4 (0.5 μg/mL), (Cat. No. A6979); (Lane C) Rabbit Anti-AtMPK6 (0.25 μg/mL), (Cat. No. A7104). The antibodies were developed with Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Arabidopsis thaliana whole leaf extract was separated by SDS-PAGE and blotted with: (Lane A) Rabbit Anti-AtMPK3 (0.5 μg/mL) (Cat. No. M8318); (Lane B) Rabbit Anti-AtMPK4 (0.5 μg/mL), (Cat. No. A6979); (Lane C) Rabbit Anti-AtMPK6 (0.25 μg/mL), (Cat. No. A7104). The antibodies were developed with Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 43 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Arabidopsis thaliana |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves |
| western blot: 2-4 μg/mL using extract (cytosolic fraction) of Nicotiana tabacum leaves |
| Application: | Anti-AtMPK3 antibody produced in rabbit has been used in immunoblotting analysis. |
| Biochem/physiol Actions: | Anti-AtMPK3 recognizes AtMPK3. |
| Biochem/physiol Actions: | AtMPK3 is a component of the plant MAPK signaling cascade that functions downstream of the FLS2 receptor and regulates the innate response to bacterial and fungal infections. AtMPK3 is also involved in the phosphorylation of VIP1 in response to Agrobacterium infection and the subsequent transfer of the bacterial T-DNA into the nucleus. RNAi mediated inhibition of AtMPK6 causes prolonged activation of AtMPK3 . Anti-AtMPK3 antibody is specific for AtMPK3 (43 kDa). The product also reacts with Nicotiana tabacum and p56 in Papaver rhoeas . In immunoblotting, staining of AtMPK3 is specifically inhibited with AtMPK3 immunizing peptide (Arabidopsis thaliana, amino acids 359-370). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein kinase AtMPK3 is a component of the MAP kinase cascade. |
| Immunogen: | Synthetic peptide corresponding to amino acids at the C-terminus of Arabidopsis thaliana MPK3. This sequence is specific to AtMPK3 (not found in the other 20 known AtMPK isoforms). It has limited homology (58% identity) to the AtMPK6 isoform and shares significant homology (67% identity, continuous epitope) with the Nicotiana tabacum ortholog WIPK. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |