Anti-MLH1 (N-terminal) antibody produced in rabbit
SIGMA/M8320 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-COCA2; Anti-FCC2; Anti-HNPCC; Anti-MGC5172
Product Type: Chemical
Immunoprecipitation Various amounts of Rabbit anti MLH1 N-terminal antibody (M8320) were used to immunoprecipitate MLH1 from MCF-7 cell lysates. Detection antibody: Monoclonal antibody to MLH1. Lanes 1. 15 μg of antibody 2. 5 μg of antibody 3. 1 μg of antibody 4. Negative control – without cell lysate 5. Negative control – without IP antibody 6. Positive control – Total MCF-7 cell lysate direct labeling
Immunofluorescence MCF7 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton X-100. Fixed cells were stained with 5 μg/mL Anti-MLH1 (N-terminal) antibody produced in Rabbit (Cat. No. M8320) followed by 1:500 Anti-Rabbit IgG-Cy3 antibody. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 80-85 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 2.5-5 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton |
| western blot: 0.5-1 μg/mL using Jurkat cell lysates | |
| UniProt accession no. | P40692  |
| Application: | • Anti-MLH1 (N-terminal) antibody produced in rabbit has been used in: • immunoblotting • immunocytochemistry |
| Biochem/physiol Actions: | MutL homolog 1 (MLH1) has been shown to be involved in stimulating carcinogenesis in the colon. |
| Biochem/physiol Actions: | MutL protein (MLH), a homolog of the E. coli MutL gene, is involved in DNA mismatch repair. Nonpolyposis colorectal cancer-2 is caused by a hereditary mutation in the MLH1 gene. This cancer can also be a result of hypermethylation of one MLH1 allele in somatic cells (a germline epimutation). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | MLH1 (N-terminal) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BASC (BRCA1-associated genome surveillance complex). |
| General description: | MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is localized on chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa. |
| General description: | MutL homolog 1 (MLH1) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors and signal transducers, named BRCA1-associated genome surveillance complex (BASC). |
| Immunogen: | synthetic peptide corresponding to amino acids 60-75 of human MLH1, conjugated to KLH via an N-terminal added cysteine residue. The corresponding peptide sequence is conserved in human, rat, and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |