Anti-MCH antibody produced in rabbit
SIGMA/M8440 - IgG fraction of antiserum, buffered aqueous solution
Synonym: MCH Antibody - Anti-MCH antibody produced in rabbit; Mch Antibody; Anti-
MDL Number: MFCD03791841
Product Type: Chemical
Immunohistochemistry
Staining of mouse lateral hypothalamus with Anti-Melanin Concentrating Hormone (MCH) (Cat. No. M8440) at a 1:200 dilution. Cells with neuronal morphology and axonal fibers were observed. Developed with Goat Anti-Rabbit IgG-Biotin, ExtrAvidin™ Peroxidase (Cat. No. E2886) and DAB/Nickel substrate.
Dr. S. Shoham, Herzog Hospital Research Lab., Jerusalem. Israel.
Staining of mouse lateral hypothalamus with Anti-Melanin Concentrating Hormone (MCH) (Cat. No. M8440) at a 1:200 dilution. Cells with neuronal morphology and axonal fibers were observed. Developed with Goat Anti-Rabbit IgG-Biotin, ExtrAvidin™ Peroxidase (Cat. No. E2886) and DAB/Nickel substrate.
Dr. S. Shoham, Herzog Hospital Research Lab., Jerusalem. Israel.
Immunohistochemistry
Staining of mouse ventromedial hypothalamus with Anti-Melanin Concentrating Hormone (MCH) (Cat. No. M8440) at a 1:200 dilution. Cells with neuronal morphology and axonal fibers were observed. Developed with Goat Anti-Rabbit IgG-Biotin, ExtrAvidin™ Peroxidase (Cat. No. E2886) and DAB/Nickel substrate.
Dr. S. Shoham, Herzog Hospital Research Lab., Jerusalem. Israel.
Staining of mouse ventromedial hypothalamus with Anti-Melanin Concentrating Hormone (MCH) (Cat. No. M8440) at a 1:200 dilution. Cells with neuronal morphology and axonal fibers were observed. Developed with Goat Anti-Rabbit IgG-Biotin, ExtrAvidin™ Peroxidase (Cat. No. E2886) and DAB/Nickel substrate.
Dr. S. Shoham, Herzog Hospital Research Lab., Jerusalem. Israel.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:2,500 |
| microarray: suitable | |
| UniProt accession no. | P20382  |
| Application: | Anti-MCH antibody may be used at a working dilution of 1:30000 for RIA. For immunohistochemistry of mouse hypothalamus sections, a working antibody dilution of 1:200 may be used. The antibody has been used for immunoblotting in cytoplasmic extracts of T cells at a concentration of 5.7 μg/ml. The antibody is suitable for protein microarray. |
| Application: | Anti-MCH antibody produced in rabbit has been used in radioimmunoassay and immunohistochemistry. |
| Biochem/physiol Actions: | MCH mRNA is overexpressed and up-regulated during fasting in the hypothalamus of ob/ob mice as well as rats. Injection of MCH into the lateral ventricles in rats promote increased food consumption. In addition, knockout mice lacking MCH gene are lean and hypophagic and show an increase in metabolic rate. In contrast, overexpression of MCH in transgenic mice leads to obesity and insulin resistance. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Melanin-concentrating hormone (MCH) is a circulating 19-amino acid residue cyclic neuropeptide present in hypothalamus. In mammals, MCH is a, encoded by the precursor pro-melanin concentrating hormone (PMCH). It was first discovered in fish and subsequently in mammalian (rat). MCH perikarya is confined largely to the lateral hypothalamus area (LHA) and the adjacent zon incerta with extensive neuronal projections throughout the brain. MCH is detected in basal ganglia, neocortex, cerebellum, thymus, brown adipose tissue, duodenum, and testis. The structure of MCH is highly conserved among species. |
| Immunogen: | synthetic peptide corresponding to human melanin-concentrating hormone conjugated to KLH. The MCH sequence is identical in many species including rat, mouse, and pig. |
| Physical form: | Solution in phosphate buffered saline containing 15 mM sodium azide. |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |