MCDB 131 Medium
SIGMA/M8537 - With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym: 131 Medium; Medium 131; Molecular, Cellular, and Development Biology 131 medium
Product Type: Chemical
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| components | sodium pyruvate: 0.11 g/L phenol red: 0.012 g/L L-glutamine: 1.461 g/L HEPES: no glucose: 1.0 g/L (Dextro) |
| concentration | 11.6 g/L |
| form | powder |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | MCDB-131 Medium has been used: • as a component of the basal medium to culture bovine retinal endothelial cells • to culture bovine lung microvascular endothelial cells • for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells |
| General description: | MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). |
| Preparation Note: | Formulated to contain 11.6 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 1.18 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |