Monoclonal Anti-MDM2 antibody produced in mouse
SIGMA/M8558 - ~2 mg/mL, clone MD-219, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD03792424
Product Type: Chemical
Western Blotting Whole extract of transfected 293T (Human embryonal kidney) cells expressing Mouse MDM2 were separated on SDS-PAGE and probed with Monoclonal Anti-MDM2 antibody produced in Mouse, Clone: MD-219 (Cat. No. M8558). The antibody was developed with 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 0.5 μg/mL antibody 2. 2 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MD-219, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 90 kDa |
| shipped in | dry ice |
| should not react with | human |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-2 μg/mL using whole extract of transfected 293T (human embryonal kidney) cells, expressing mouse MDM2 | |
| UniProt accession no. | P23804   |
| Biochem/physiol Actions: | In addition, to regulation MDM2 also mediates the degradation of p53. MDM2 protein has been shown to activate cell proliferation by stimulating the S-phase-inducing transcription factors, E2F1/DP1. Overexpression of MDM2 results in reduced quantities of co-expressed p53, and disruption of the p53-MDM2 interaction by mutation results in both activation and accumulation of p53. The amplification of the MDM2 gene has been demonstrated in various human neoplasms. MDM2 is frequently overexpressed in the nuclei of soft-tissue sarcomas, and to a much lesser extent in a wide variety of malignancies, such as bladder, breast, testes, and cervix cancers, and in glial cells, and a range of hematological disorders. Monoclonal antibody reacting specifically with MDM2 is an essential tool in defining the interactions and distributions of MDM2, its function in signaling pathways and in mutual regulation of p53. |
| Biochem/physiol Actions: | The antibody shows predominantly nuclear and some cytoplasmic staining in cells. |
| Biochem/physiol Actions: | Transformed mouse 3T3 cell double minute 2 (MDM2) acts as a negative regulator of the tumor suppressor p53. It also regulates the cell cycle and inflammatory activities. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mouse 3T3 cell double minute 2 (MDM2) gene was originally found on mouse double minute chromosomes. |
| General description: | Transformed mouse 3T3 cell double minute 2 (MDM2) is an E3 ligase expressed in podocytes. It is overexpressed in lymphomas. |
| Immunogen: | recombinant mouse MDM2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |