Anti-Mouse IgG (whole molecule) antibody produced in goat
SIGMA/M8645 - affinity isolated antibody, lyophilized powder
Synonym: Goat Anti-Mouse IgG; Goat IgG Antibody
MDL Number: MFCD00162638
Product Type: Chemical
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| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | Ouchterlony double diffusion: suitable |
| Application: | Anti-mouse IgG (whole molecule) antibody (diluted 1:20) can be used in Indirect immunofluorescence. It can also be used as a secondary antibody in western blotting to release NC1 domain of type IV collagen. Further, it can also be used in enzyme immunoassays (50 μgram) . |
| Application: | Anti-mouse IgG (whole molecule) antibody (diluted 1:20) can be used in: • indirect immunofluorescence • western blotting to release NC1 domain of type IV collagen • enzyme immunoassays (50 μgram) |
| Biochem/physiol Actions: | IgG regulates several functions such as phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-mouse IgG (whole molecule) antibody can be used in T cell proliferation assays. It can also be used in Ouchterlony Double Diffusion and immunoelectrophoresis. Goat anti- mouse IgG (whole molecule) antibody reacts specifically with mouse IgG subclasses IgG1, IgG2a, IgG2b and IgG3. The product has also shown no reactivity to human serum proteins. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. |
| Immunogen: | IgG purified from pooled normal mouse serum. |
| Other Notes: | Antibody adsorbed with human serum proteins |
| Physical form: | Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2 |
| Preparation Note: | Reconstitute with 1 mL of 0.135 M sodium chloride. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |