Magnesium chloride solution
SIGMA/M8787 - PCR Reagent, 25 mM MgCI2 solution for PCR
Synonym: PCR optimization
CAS Number: 7786-30-3
Empirical Formula (Hill Notation): Cl2Mg
Molecular Weight: 95.21
MDL Number: MFCD00011106
Linear Formula: MgCl2
Product Type: Chemical
| application(s) | agriculture diagnostic assay manufacturing |
| color | colorless |
| concentration | 25 mM±1 mM |
| foreign activity | DNase, RNase, none detected |
| form | liquid |
| InChI | 1S/2ClH.Mg/h2*1H;/q;;+2/p |
| InChI key | TWRXJAOTZQYOKJ-UHFFFAOYSA |
| packaging | vial of 1.5 mL |
| Quality Level | 200  |
| 300 |
|
| SMILES string | Cl[Mg]Cl |
| storage temp. | 2-8°C |
| technique(s) | PCR: suitable |
| Application: | Magnesium chloride solution has been used as a component of the: • amplification mixture for polymerase chain reaction (PCR) • amplification mixture in an inter simple sequence repeats (ISSR)-PCR • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP) • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA • reaction mix for RT-PCR to assess the gene expression |
| Application: | Suitable for optimization of polymerase chain reactions. |
| General description: | Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352302 |