Anti-FLAG® M2 Magnetic Beads
SIGMA/M8823 - affinity isolated antibody
Synonym: Monoclonal ANTI-FLAG® M2 antibody produced in mouse; FLAG® magnetic affinity resin; FLAG® resin for high throughput; Flag® Affinity resin; Anti-ddddk; Anti-dykddddk
Product Type: Chemical
Immunopreciptiation Anti-FLAG® M2 Magnetic Beads: Cat. No. M8823: Binding Capacity of the Anti-FLAG® M2 Magnetic Beads. Western blot analysis demonstrates that binding capacity of the Anti-FLAG® magnetic beads when compared to the equivalent agarose conjugate (Cat. No. A2220). Courtesy of Drs. Guillaume Adelmant and Jarrod Marto, Blais Proteomics Center, Dana-Farber Cancer Institute, 2009.
Immunopreciptiation Anti-FLAG® M2 Magnetic Beads: Cat. No. M8823: Purification of Two Different FLAG®-tagged Baits. Two different FLAG®-tagged baits were purified either from a HeLa-S3 nuclear extract (Nuc. Extr.) or from a HeLa-S3 cytoplasmic fraction (Cyto. Extr.). Both samples were prepared from 10e8 cells. The purifications were preformed on the Thermo King Fisher magnetic bead processor. 10% of each FLAG® immunoprecepitation elution was loaded on the gel and silver stained. Courtesy of Drs. Guillaume Adelmant and Jarrod Marto, Blais Proteomics Center, Dana-Farber Cancer Institute, 2009.
| analyte chemical class(es) | proteins (FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag) |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| bead size | 20-75 μm |
| capacity | ≥0.6 mg/mL binding capacity |
| clone | M2, monoclonal |
| conjugate | magnetic beads |
| form | suspension |
| isotype | IgG1 |
| matrix | superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm. |
| Quality Level | 200  |
| shelf life | 2 yr at -20 °C |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | affinity chromatography: suitable |
| immunoprecipitation (IP): suitable |
| Application: | Suitable for immunoprecipitation procedures. Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide Learn more product details in our FLAG® application portal. |
| Features and Benefits: | The magnetic properties allow for: - Very rapid separation - Significantly accelerated manipulations, such as repetitive washings - Processing of multiple samples performed in plate formats This leads to: - Faster experimentation - Better reproducibility - More accurate quantitation of the proteins of interest |
| General description: | Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence. |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 1 mL in poly bottle |
| Physical form: | Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |