4-Methylumbelliferyl phosphate
SIGMA/M8883 - phosphatase substrate
Synonym: 4-
CAS Number: 3368-04-5
Empirical Formula (Hill Notation): C10H9O6P
Molecular Weight: 256.15
EC Number: 222-137-7
MDL Number: MFCD00016969
Linear Formula: C10H9O6P
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M8883-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M8883-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M8883-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M8883-5G
| assay | ≥98% (HPLC) |
| description | phosphatase substrate |
| fluorescence | λex 319 nm; λem 384 nm (pH 9.1) |
| λex 360 nm; λem 449 nm (Reaction product) | |
| form | powder |
| InChI | 1S/C10H9O6P/c1-6-4-10(11) |
| InChI key | BCHIXGBGRHLSBE-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | CC1=CC(=O)Oc2cc(OP(O)(O)= |
| solubility | water: 25 mg/mL, clear to very slightly hazy, colorless |
| storage temp. | −20°C |
| Application: | 4-Methylumbelliferyl phosphate has been used as a substrate for alkaline phosphatase in the peptide-binding assay. It has also been used as a fluorogenic substrate in enzyme-linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | 4-Methylumbelliferyl phosphate serves as a fluorogenic substrate for calmodulin-dependent protein phosphatase and in kinetic studies of alkaline phosphatase. It is a substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. 4-Methylumbelliferyl phosphate is seven times more sensitive than phenolphthalein monophosphate and 8-13 times more sensitive than with p-nitrophenyl phosphate when used in enzyme immunoassays for the detection of antibodies to human immunodeficiency viruses. |
| General description: | Fluorogenic substrate for phosphatases. |
| Packaging: | 1, 5 g in poly bottle |
| Packaging: | 100, 250 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |