Monoclonal Anti-Myocardin antibody produced in mouse
SIGMA/M8948 - ~2.0 mg/mL, clone MCR54, purified immunoglobulin
Synonym: Anti-MYCD; Anti-MYOCD
Product Type: Chemical
Immunoblotting Lysate of HEK-293T cells expressing Myocardin was separated on SDS-PAGE and probed with Mouse Anti-myocardin Clone: MCR54 (Cat. No. M8948). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 4 μg/mL antibody +10 μg/mL Myocardin (884-901) immunizing peptide 3. 4 μg/mL antibody +10 μg/mL Myocardin (39-58) immunizing peptide
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MCR54, monoclonal |
| concentration | ~2.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~150 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 2.5-5 μg/mL using HEK-293T cells overexpressing myocardin |
| UniProt accession no. | Q8IZQ8  |
| Application: | Monoclonal Anti-Myocardin antibody produced in mouse has been used in western blot analysisimmunofluorescenc |
| Biochem/physiol Actions: | Myocardin (MYOCD) takes part in the differentiation of vascular smooth muscle cells (VSMC). It is a co-factor of serum response factor (SRF) and regulates the activation of those genes which are dependent on SRF. MYOCD forms a complex with SRF on DNA. |
| Biochem/physiol Actions: | Myocardin is a smooth and cardiac muscle-specific transcriptional coactivator of serum response factor (SRF). It regulates the expression of a set of cardiac and smooth muscle-specific genes. Myocardin was implicated in the pathogenesis of atherosclerosis, Alzheimer′s disease, and malignant transformation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Myocardin (mouse IgG2b isotype) is derived from the hybridoma MCR54 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human myocardin. Myocardin (MYOCD) is a transcriptional coactivator. |
| General description: | Myocardin (MYOCD) is expressed in the muscles and cardiomyocytes. It is a co-transcriptional factor. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |