Malic Dehydrogenase from bovine heart
SIGMA/M9004 - ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)
Synonym: L-Malate:NAD+ oxidoreductase; MDH
EC Number: 232-622-5
MDL Number: MFCD00131567
Product Type: Chemical
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| biological source | bovine heart |
| form | ammonium sulfate suspension |
| impurities | ≤0.01% Glutamic-Oxalacetic Transaminase |
| ≤0.01% Glutamic-Pyruvic Transaminase | |
| mol wt | 35 kDa |
| Quality Level | 200  |
| specific activity | 2000-4000 units/mg protein (modified Warburg-Christian) |
| storage temp. | 2-8°C |
| Application: | Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas. |
| General description: | Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed. |
| General description: | Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle. |
| Other Notes: | One unit will convert 1.0 μmole of oxalacetate and β-NADH to |
| Physical form: | Suspension in 3 M (NH4)2SO4 - 0.01 M KH2PO4 solution, pH 7.3 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 2000-4000 units/mg protein (modified Warburg-Christian) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.37 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |