Malachite Green oxalate salt
SIGMA/M9015 - certified by the BSC
Synonym: N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate; Basic Green 4
CAS Number: 2437-29-8
Empirical Formula (Hill Notation): C23H25N2 · C2HO4 · 0.5C2H2O4
Molecular Weight: 463.50
EC Number: 219-441-7
Linear Formula: C23H25N2 · C2HO4 · 0.5C2H2O4
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M9015-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M9015-25G
| agency | certified by the BSC |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | faint green to very dark green |
| composition | Dye content, ≥90% |
| form | powder or crystals |
| InChI | 1S/C23H25N2.C2H2O4/c1-24( |
| InChI key | REZFCYOVFCJTDG-UHFFFAOYSA |
| mp | 144-150 °C |
| Quality Level | 200  |
| SMILES string | C[N+](C)=C1C=CC(C=C1)=C(C |
| solubility | H2O: 1 mg/mL, blue to very deep blue |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | Certified for use as a counterstain for paraffin embedded botanical material; as a spore stain for bacteria by the method of Schaeffer and Fulton. |
| Application: | Malachite Green oxalate salt has been used for phosphate quantification. |
| Application: | Malachite Green oxalate salt has been used: • in the preparation of Alexander staining solution for cell viability test • in malachite green-ammonium molybdate assay for determining the adenosine triphosphatase (ATPase) activity of binding immunoglobulin protein (BiP) • as a hydrophobic ion, to study the label-free molecular transport in the living cell membranes of Escherichia coli in real time by second-harmonic generation (SHG) method |
| General description: | Malachite Green is a basic dye belonging to the triphenylmethane group. It is required in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate. |
| Other Notes: | The molecular mass of malachite green oxalate is expressed as either: 927.00 g/mol - the mass of 2 mol malachite green + 2 mol monohydrogen oxalate + 1 mol dihydrogen oxalate, or 463.50 g/mol - the mass of 1 mol malachite green + 1 mol monohydrogen oxalate + 0.5 mol dihydrogen oxalate |
| Packaging: | 25, 100 g in glass bottle |
| Symbol |     GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 - H318 - H361d - H410 |
| Precautionary statements | P201 - P273 - P280 - P301 + P310 + P330 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xn,N |
| Risk Statements | 63-22-41-50/53 |
| Safety Statements | 26-36/37-39-46-60-61 |
| RIDADR | UN2811 - class 6.1 - PG 3 - EHS - Toxic solids, organic, n.o.s., |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 144-150 °C |
| Storage Temp. | room temp |
| Colour Index Number | 42000 |
| UNSPSC | 12171500 |