Macrophage Colony-Stimulating Factor from mouse
SIGMA/M9170 - M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: mCSF-1; mM-CSF; CSF-1; M-CSF
CAS Number: 81627-83-0
EC Number: 214-684-5
MDL Number: MFCD00166591
Product Type: Chemical
Bioassay EC50 is defined as the effective concentration of M-CSF that elicits a 50% attachment factor in cell based bioassay.
SDS-PAGE The purity of recombinant Mouse M-CSF (Cat. No. M9170) was determined by SDS-PAGE of reduced (+) and non-reduced (-) M-CSF and staining with Coomassie Blue.
| assay | ≥98% (SDS-PAGE) |
| biological source | mouse |
| color | white |
| form | lyophilized powder |
| impurities | ≤1.000 EU/μg |
| mol wt | dimer 18.2 kDa (containing 156 amino acid residues) |
| packaging | pkg of 10 μg |
| potency | 0.500-3.000 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P07141  |
| Analysis Note: | The proliferative activity is tested by the dose-dependent stimulation of the murine monocytic cell line, M-NFS-60. |
| Application: | Macrophage Colony-Stimulating Factor from mouse has been used: • in the initiation of osteoclastogenesis in bone marrow monocyte cells • to stimulate osteoclast differentiation in non-adherent hematopoietic cell • for the generation of blood-derived stem cells |
| Biochem/physiol Actions: | Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by a number of cells, including fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF can stimulate the proliferation of isolated macrophages, augment the production and release of cytokines and other inflammatory modulators from macrophages, enhance macrophage antibody-dependent cell-mediated cytotoxicity, prime and enhance macrophages in their ability to kill tumor cells and other microorganisms, stimulate pinocytosis, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing of a single M-CSF gene and due to variations in glycosylation, the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Human and mouse M-CSF share approximately 80% sequence homology in the conserved N-terminal region of 150 amino acids. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions. |
| Biochem/physiol Actions: | Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF augments many of the physiological functions of macrophages, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing and glycosylation the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions. |
| Physical form: | Lyophilized from a sterile filtered buffered aqueous solution. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P261 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |