3-Methyladenine
SIGMA/M9281 - autophagy inhibitor
Synonym: 3-MA; 6-
CAS Number: 5142-23-4
Empirical Formula (Hill Notation): C6H7N5
Molecular Weight: 149.15
EC Number: 225-908-6
MDL Number: MFCD00010531
Linear Formula: C6H7N5
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M9281-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: M9281-500MG
| assay | ≥99% (HPLC) |
| biological source | synthetic (organic) |
| form | powder |
| InChI | 1S/C6H7N5/c1-11-3-10-5(7) |
| InChI key | FSASIHFSFGAIJM-UHFFFAOYSA |
| mp | ~300 °C (dec.) (lit.) |
| Quality Level | 300  |
| SMILES string | Cn1cnc(N)c2ncnc12 |
| solubility | DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow |
| storage temp. | room temp |
| Application: | 3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM. |
| Application: | 3-Methyladenine has been used as an autophagy inhibitor: • to determine the expressions of autophagy-related genes (Beclin 1, light chain 3 (LC3) and p62) using the transfected human bone marrow mesenchymal stem cells (BM-MSCs) • with cisplatin to study the inhibition of autophagy influenced cisplatin-induced apoptosis in A2780cp cells • to study the interplay between colistin-induced autophagy and apoptosis |
| Application: | Standard for detection of nucleic acid methylation during carcinogenesis. |
| Biochem/physiol Actions: | 3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K). |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 100, 500 mg in poly bottle |
| Preparation Note: | This product is soluble in DMF (10 mg/mL; may require gentle heating). It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/mL with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA may be prepared in DMSO at 100 mM. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (HPLC) |
| mp | ~300 °C (dec.) (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 41106305 |