Anti-MeCP2 antibody produced in rabbit
SIGMA/M9317 - ~0.6 mg/mL, affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD02686372
Product Type: Chemical
Western Blotting Whole nuclear extracts of the MCF7 breast carcinoma cell line were separated on SDS-PAGE and probed with Anti-MeCP2 antibody produced in Rabbit (Cat. No. M9317). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~0.6 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~75 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 0.5-1 μg/mL using nuclear extracts of the MCF7 breast carcinoma cell line. | |
| UniProt accession no. | P51608  |
| Biochem/physiol Actions: | MeCP2 can bind to a single symmetrically methylated CpG pair. It is also capable of binding to chromosomes at sites known to have methylated DNA. MeCP2 silences transcription by recruiting the histone deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor thus removing acetyl groups from histones and consequently silencing genes. |
| Biochem/physiol Actions: | Methyl-CpG binding protein 2 (MeCP2) has been shown be crucial for brain development. It is part of the methyl-CpG-binding proteins, which are involved in repression of gene expression by binding to methylated DNA. Mutations in the gene encoding MeCP2 have been associated with Rett syndrome (RTT). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Methyl-CpG binding protein 2 (MeCP2) possesses a methyl-CpG-binding domain (MBD) and a transcriptional repression domain. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of human MeCP2 (amino acids 465-478) conjugated to KLH. The sequence is conserved in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |