β-Mannosidase from Helix pomatia
SIGMA/M9400 - 5-30 units/mL, ammonium sulfate suspension, crude extract
Synonym: β-D-Mannoside mannohydrolase
CAS Number: 9025-43-8
MDL Number: MFCD00130638
Product Type: Chemical
Figure 1. Exoglycosidase enzymes commonly used to determine the structure of N-linked glycans by sequential degradation.
| concentration | 5-30 units/mL |
| foreign activity | β-N-acetylglucosaminidase and α-mannosidase <1.0% |
| form | ammonium sulfate suspension |
| mol wt | 94 kDa |
| quality | crude extract |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | β-Mannosidase from Helix pomatia has been used: • in the digestion of glycans for enzymatic analysis • in the treatment of labeled monogalactosyl Manno-oligosaccharides • in non-mammalian glycosidase inhibition assays |
| Biochem/physiol Actions: | β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements. |
| General description: | β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2. |
| Other Notes: | One unit will hydrolyze 1 μmole of p-nitrophenyl-β- |
| Physical form: | Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.25 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |