Mannitol Dehydrogenase from Leuconostoc mesenteroides
SIGMA/M9532 - lyophilized powder, ≥60 units/mg protein
Synonym: polyol dehydrogenases; Mannitol:NAD oxidoreductase
CAS Number: 9001-65-4
MDL Number: MFCD00131575
Product Type: Chemical
Mechanism of Mannitol Dehydrogenase: D(-)Fructose + β-NADH → Mannitol Dehydrogenase→ D-Mannitol + β-NAD
| form | lyophilized powder |
| mol wt | 136 kDa |
| Quality Level | 200  |
| specific activity | ≥60 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Assayed according to the method of Yamanaka, Meth. Enzymol., 41(B), 138 (1975). |
| Application: | Can be used for the production of D-mannitol. |
| Application: | It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers. |
| Application: | This preparation is useful in the determination of mannitol in urine. |
| Biochem/physiol Actions: | Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of |
| General description: | Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size. |
| General description: | Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol. |
| General description: | The molecular weight of mannitol dehydrogenase is 136 kDa. |
| Other Notes: | One unit will reduce 1.0 μmole of |
| Physical form: | Lyophilized powder containing buffer salts, potassium phosphate, and dithiothreitol |
| Preparation Note: | Partially purified |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥60 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.1.67 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |